Wednesday
March 3, 1999
Welcome
Lester Packer
President, Oxygen Club of California
Department of Molecular and Cell Biology
University of California, Berkeley
Keynote Lecture
Dedicated to Lars Ernster
1920 1998
Chairperson Paul Hochstein
Department of Molecular Pharmacology & Toxicology
School of Pharmacy, University of Southern California, Los Angeles,
California
06:00 07:00 Carotenoid metabolites as cell signaling modulators
Norman I. Krinsky
Department of Biochemistry, School of Medicine
Tufts University, Boston, Massachusetts
07:00 Welcome Reception
Chairperson William A. Pryor
Biodynamics Institute, Louisiana State University
Baton Rouge, Louisiana
09:00 09:20 Formation and biologically relevant reactions of
ONOO
Wim Koppenol
Laboratorium für Anorganische Chemie, ETHZ, Zürich, Switzerland
09:20 09:40Mechanisms of the antioxidant action of nitric oxide
Victor Darley-Usmar
Department of Pathology, University of Alabama at Birmingham
Birmingham, Alabama
09:40 10:00 Reactions of .NO, .NO2, and ONOO in membranes:
Physiological Implications
R. Kalyanaraman
Biophysics Research Institute, Medical College of Wisconsin
Milwaukee, Wisconsin
10:00 10:20 Nitric oxide in ischemia and reperfusion injury:
Is NO protective and injurious?
David Wink
Radiation Biology Branch, National Cancer Institute
National Institutes of Health, Bethesda Maryland
10:20 10:40The reactions of ubiquinol with .NO and ONOO and
the regulation of mitochondrial respiration
Alberto Boveris
Department of Physical Chemistry, School of Pharmacy and Biochemistry
University of Buenos Aires, Buenos Aires, Argentina
10:40 11:00 Coffee Break
Session II Thiols in Biochemistry, Cell Regulation, and
Medicine
Chairpersons Mulchand Patel
Department of Biochemistry, School of Medicine and Biomedical Sciences
State University of New York at Buffalo, Buffalo, New York
Hans-Jürgen Tritschler
Asta Medica AWD, Frankfurt, Germany
11:00 11:20Are there functional roles for methionine oxidation in
proteins?
Rodney L. Levine
Laboratory of Biochemistry, National Heart, Lung, and Blood Institute
National Institutes of Health, Bethesda, Maryland
11:20 11:40Redox regulation of GCS subunit gene expression:
Role of Nrf2 and small maf proteins in gene induction
by PDTC
Timothy Mulcahy
Department of Human Oncology, University of Wisconsin Medical School
Madison, Wisconsin
11:40 12:00The effects of overexpression of glutamate-cysteine
ligase on cell survival, cell growth, and apoptosis
Terrance J. Kavanagh
Department of Environmental Health, University of Washington
Seattle, Washington
12:00 12:20Lipoate differentially regulates agonist-induced
ICAM-1 and VCAM-1 gene expression in human
endothelial cells
Sashwati Roy
Department of Molecular and Cell Biology, University of California
Berkeley, California
12:20 12:40A comparison of the effect of a-lipoic acid and a
tocopherol supplementation on plasma, LDL, and
whole body oxidation
Kenny Jialal
Center for Human Nutrition, Department of Clinical Nutrition
The University of Texas Southwestern Medical Center at Dallas
Dallas, Texas
05:00 05:15Redox cycles of caffeic acid during the oxidation of
LDL
João Laranjinha
Laboratorio de Bioquimica, Faculdade de Farmacia and Centro de
Neurociencias, Universidade de Coimbra, Portugal
05:15 05:30Repair of iron-induced DNA oxidation by the flavonoid
myricetin in hepatocyte culture
Isabelle Morel
Laboratoire de Biologie Cellulaire et Végétale
Faculté de Pharmacie, Universite de Rennes 1, Rennes, France
05:30 05:45 Modulation of gene expression by phenolic
antioxidants ‹ The epigallocatechin example
Rex Tyrrell
Department of Pharmacy and Pharmacology, University of Bath
Bath, United Kingdom
05:45 06:00French maritime pine bark extract induces changes in
gene expression profile in human keratinocytes
Bertrand Rihn
Department of Molecular and Cell Biology
University of California, Berkeley, California
06:00 06:15Flavonoids as antioxidant cardioprotective agents:
from molecular pharmacology to the clinic
Aalt Bast
Department of Pharmacology, Faculty of Medicine
University of Maastrich, Maastricht, The Netherlands
06:30 Poster Viewing and Refreshments
09:20 09:40 Oxidized lipid-cell interactions
Sam Parthasarathy
Department of Gynecology and Obstetrics
Emory University School of Medicine, Atlanta, Georgia
09:40 10:00Selective oxidation of methionine residues enhances
rather than decreases potential anti-atherogenic
properties of apolipoprotein A-I
Roland Stocker
Cell Biology Group, The Heart Research Institute
Camperdown, NSW, Australia
10:00 10:20Apolipoprotein A-I removes seeding molecules that
are required for oxidation from freshly isolated
normal LDL: Increased levels in atherogenesis
Mohamad Navab
Cardiology and Cardiothoracic Surgery, University of California
Los Angeles, California
10:20 10:40 Coffee Break
10:40 11:00Modulation of vascular smooth muscle cell function
by lipoprotein oxidation products
Guy Chisolm
Department of Cell Biology, Cleveland Clinic Foundation, Ohio
11:00 11:20Molecular mechanism of the effect of homocysteine
in the development of atherosclerosis
Nesrin Kartal Özer
Department of Biochemistry, Faculty of Medicine
Marmara University, Istanbul, Turkey
11:20 11:40Oxidative mechanisms of radiation-induced
atherosclerosis
Diane Tribble
Department of Molecular and Nuclear Medicine
Ernest Orlando Lawrence Berkeley National Laboratory
University of California, Berkeley, California
11:40 12:00Antioxidant vitamins and the pursuit to prove efficacy
in preventing atherosclerosis in humans
Howard Hodis
Atherosclerosis Research Unit, School of Medicine
University of Southern California, Los Angeles, California
Session VI Round Table Discussion
Natural Antioxidants: Health Effects
12:00 01:00
Coordinators Norman I. Krinsky
Lester Packer
Panel Members Angelo Azzi
Gladys Block
Carroll E. Cross
Manfred Dunker
Osmo Hänninen
William A. Pryor
Catherine Rice-Evans
Helmut Sies
01:00 02:00 Lunch
02:00 02:20 Biomarkers of oxidative stress in the nervous system
Barry Halliwell
Neurodegenerative Disease Research Centre, King's College
London, United Kingdom
02:20 02:40 Micronutrient deficiency and the brain
Bruce N. Ames
Department of Molecular and Cell Biology, University of California
Berkeley, California
02:40 03:00The proteasome and degradation of oxidized proteins
during cellular senescence
Tilman Grune
Klinik für Physikalische Medizin, Medizinische Fakultät
Humboldt- Universität zu Berlin, Berlin, Germany
03:00 03:20 Protein oxidative damage during aging is selective and
not random
Raj Sohal
Department of Biological Sciences, Southern Methodist University
Dallas, Texas
03:20 03:40The iron-binding protein ferritin protects vulnerable
dopaminergic neurons against neurodegeneration
associated with a toxic model of Parkinson's disease
Julie Andersen
Andrus Gerontology Center, University of Southern California
Los Angeles, California
03:40 04:00Mitochondria abnormalities and metabolic
dysfunction in Alzheimer disease
Mark Smith
Institute of Pathology, Case Western Reserve University
Cleveland, Ohio
04:00 04:20 Coffee Break
Session VIII Roles of Mitochondria in Neurodegeneration and
Aging
Chairperson Gino Cortopassi
Department of Molecular Biosciences, University of California
Davis, California
Huber R. Warner
National Institute on Aging, National Institutes of Health
Bethesda, Maryland
04:20 04:40 Absorption and effects on mitochondrial activity of
oral ubiquinone in Parkinsonian patients
M. Flint Beal
Department of Neurology, Cornell University Medical College
New York, New York
04:40 05:00 Intracellular events in glutamate-induced death of
HT4 neuronal cells
Chandan K. Sen
Environmental Energy Technologies Division
Ernest Orlando Lawrence Berkeley National Laboratory
University of California, Berkeley, California
05:00 05:20 Modulation of Bcl-2 and Bax expression in nitric
oxide-induced apoptosis in cultured cells
Hugo Monteiro
Fundaçao Pro-Sangue Hemocentro, Sao Paulo, Brazil
05:20 05:40Oxidant sensitivity of Friedreich's ataxia cells
Gino A. Cortopassi
Department of Molecular Biosciences, University of California
Davis, California
05:40 06:00The mitochondrial-lysosomal axis theory of cellular
aging and the autophagocytic capacity of lipofuscin
loaded cells
Ulf Brunk
Department of Pathology, University of Linköping, Linköping, Sweden
Oral Presentation of Selected Posters
06:00 07:00
Coordinators Norman I. Krinsky
Department of Biochemistry, School of Medicine
Tufts University, Boston, Massachusetts
Catherine Rice-Evans
International Antioxidant Research Centre
Division of Biochemistry and Molecular Biology
UMDS Guy's Hospital, London, United Kingdom
_________________________________________
Award Selection Committee
Josiane Cillard
Laboratoire de Biologie Cellulaire et Végétale
Faculté de Pharmacie
Universite de Rennes 1, Rennes, France
Carroll E. Cross
Division of Pulmonary-Critical Care Medicine,
Department of Medicine and Physiology, University of California, Davis
R. Kalyanaraman
Biophysics Research Institute, Medical College of Wisconsin
Milwaukee, Wisconsin
Takashi Okamoto
Department of Molecular Genetics
Nagoya City University Medical School, Nagoya, Japan
Roland Stocker
Cell Biology Group, The Heart Research Institute
Camperdown, NSW, Australia
Rex Tyrrell
Department of Pharmacy and Pharmacology, University of Bath
Bath, United Kingdom
07:30 Banquet
Awards Presentation
Chairperson Enrique Cadenas
Department of Molecular Pharmacology & Toxicology
School of Pharmacy, University of Southern California, Los Angeles
Richard F. Thompson
Program for Neural, Informational, and Behavioral Sciences
Hedco Neurosciences Building, University of Southern California
Los Angeles, California
09:00 09:20Neuroprotective and neurotrophic estrogens:
Implications for prevention and treatment of
neurodegenerative disease
Roberta Diaz Brinton
Department of Molecular Pharmacology & Toxicology
School of Pharmacy, University of Southern California, Los Angeles
09:20 09:40Oxidative stress as a mechanism in glial activation
during aging
Caleb Finch
Ethel Percy Andrus Gerontology Center
University of Southern California, Los Angeles, California
09:40 10:00The role of neuroinflammatory processes in brain
aging and neurodegeneration
Robert A. Floyd
Free Radical Biology and Aging Research Program
Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma
10:00 10:20 Mechanisms of protection against apoptosis in
neurodegeneration
James D. Adams, Jr.
Department of Molecular Pharmacology & Toxicology
School of Pharmacy, University of Southern California, Los Angeles
10:20 10:40 Is lipofuscin a proven marker of oxidative stress?
Eduardo Porta
Department of Pathology, School of Medicine, University of Hawaii
Hawaii, Honolulu
10:40 11:00Attenuation of the age-dependent accrual of oxidative
damage in rhesus monkey skeletal muscle by dietary
restriction
Richard Weindruch
Department of Medicine, University of Wisconsin , VA Hospital
Madison, Wisconsin
11:00 11:20 Coffee Break
Session X Selegiline and Neurodegeneration
Chairperson Helmut Sies
Institut für Physiologische Chemie I, Heinrich-Heine Universität
Düsseldorf, Germany
11:20 11:40Selegiline can mediate neuronal rescue rather than
neuronal protection
William G. Tatton
Department of Neurology, Mount Sinai School of Medicine
New York, New York
11:40 12:00A role for antioxidants in Alzheimer's disease
treatment: selegiline and vitamin E trial
Mary Sano
Department of Neurology, College of Physicians and Surgeons
Columbia University, New York
12:00 12:20 Effects of selegiline in patients with Parkinson's
disease
C. Warren Olanow
Department of Neurology, Mount Sinai School of Medicine
New York, New York
12:20 12:40a-Selegiline and understanding cell death in
Parkinson's patients
Peter Jenner
Neurodegenerative Disease Research Centre, Pharmacology Group
King's College, London, United Kingdom
Concluding Remarks
Lester Packer, President, Oxygen Club of California
Department of Molecular and Cell Biology
University of California, Berkeley, California
01:00 Farewell Lunch